ACTIVITY:  Application of Instructional methodology by students on DNA Isolation

   

FACULTY NAME: RAKESH N R                                              DATE: 16/11/2017

                                                             

SUBJECT CODE: 15BTL47                                                        SEM/BRANCH: V/ BT

SUBJECT NAME:  Cell and Molecular Biology                         ACADEMIC YEAR: 2017-2018

  

About DNA Isolation by C-Tab Method

         

          Isolating DNA from plant tissues can be very challenging as the biochemistry between divergent plant species can be extreme. One option for purifying DNA using CTAB exploits that polysaccharides and DNA have different solubilities in CTAB depending on the concentration of sodium chloride.  At higher salt concentrations, polysaccharides are insoluble, while at lower concentrations DNA is insoluble.  Consequently, by adjusting salt concentration in lysates containing CTAB, polysaccharides and DNA can be differentially precipitated.  

Outcome of the Activity, students are able to:

·         Students were able to Demonstrate the Isolating DNA from plant tissues

·         Understand the Principles of  DNA Precipitation by Ethanol 

·         Application of C-Tab Method of Isolating DNA from different sources